. 2009 Jun 15;20(12):2820–2830. doi: 10.1091/mbc.E09-01-0069

Table 4.

FRAP analysis of Kar3-GFP at microtubule plus ends near the shmoo tip

	k (rate of dissociation), a.u	t_1/2, s	R, % recovery
Wild type, glucose	0.104 ± 0.026	6.99 ± 1.61	58 ± 18
Wild type, galactose	0.115 ± 0.037	6.66 ± 3.03	62 ± 17
GAL1-GPA1 (on)^a	0.050 ± 0.015⁺	14.86 ± 3.76⁺⁺	63 ± 14
GAL1-GPA1 (off)^b	0.094 ± 0.027	8.03 ± 2.24	60 ± 20
GAL1^EG43-GPA1/gpa1Δ (off)^b	0.156 ± 0.047^*	4.76 ± 1.17^*	63 ± 16
GAL1^EG43-GPA1/gpa1Δ (on)^a	0.097 ± 0.024	7.29 ± 1.52	56 ± 19
gpa1^ts, 37°C	0.222 ± 0.078^**	3.35 ± 2.64⁺	49 ± 23
gpa1^ts, 25°C	0.113 ± 0.067	6.79 ± 2.47	62 ± 16

All values are reported as the mean ± SD, n = 10 for each measurement.

*p ≤ 0.01, **p ≤ 10⁻³, ⁺p ≤ 10⁻⁴, ⁺⁺p < 10⁻⁵; The statistical comparisons are as follows: Cells overexpressing Gpa1 (lines 3 and 5) and cells deficient in Gpa1 (lines 4 and 6) vs. wild-type cells cultured in the corresponding medium (glucose or galactose), and gpa1^ts cells at 23°C vs. gpa1^ts cells at 37°C (lines 7 and 8).

^a Galactose medium.

^b Glucose medium.