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. 2009 Jun 15;20(12):2831–2840. doi: 10.1091/mbc.E08-09-0911

Figure 6.

Figure 6.

Rab7 activation reverses the growth factor-independent survival of PKCδ-deficient cells. (A) Cell lines stably expressing both DN-PKCδ and Rab7-Q67L were generated. GFP-Rab7-Q67L levels were determined by flow cytometry. The mean of three independent experiments is shown. Error bars, SD. (B) Equivalent DN-PKCδ expression was confirmed by Western blotting with anti-HA antibodies. (C) Growth factor withdrawal was performed on the clones evaluated in A and B. Cell viability was determined by vital dye exclusion and flow cytometry. A representative experiment of three is shown. Error bars, SD. (D) GFP-Rab7-Q67L was stably expressed the PKCδ RNAi-1 cell line (see Figure 5E). Mean GFP-Rab7-Q67L expression level from three experiments is expressed on the same scale as in Figure 7A. (E) PKCδ knockdown was confirmed in the clones evaluated in D by Western blotting for PKCδ. (F) The clones examined D and E were withdrawn from growth factor, and cell viability was determined by vital dye exclusion and flow cytometry. A representative experiment of three independent experiments is shown. Error bars, SD.