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. 2009 Jun 15;20(12):2864–2873. doi: 10.1091/mbc.E09-02-0168

Figure 6.

Figure 6.

WSX-1 and CNTFR are essential for HN-mediated protection against Aβ42-induced death of SH-SY5Y cells expressing p75NTR. SH-SY5Y cells, transfected with 1.0 μg of the pFLAG vector (Vec) or pFLAG-human p75NTR, were replated onto 96-well dishes at 3 h after transfection. At 24 h after the start of transfection, they were coincubated with 1 μM Aβ42 together with or without 10 μM HN. One microliter of 1 mg/ml neutralizing goat polyclonal antibody mAb to human CNTFR or control goat IgG was added in A, and 1 μL of rabbit polyclonal antibody to the N-terminal 16 peptide of human WSX-1 or preimmune serum was added in B. At 48 h after the start of transfection, the cell viability was determined with calcein-staining assays. Microscopic pictures of the cells were also taken. DDW, distilled water. * p < 0.05; ** p < 0.01; *** p < 0.001; n.s., not significant.