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. 2009 Jun 15;20(12):2874–2884. doi: 10.1091/mbc.E08-12-1177

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© 2009 by The American Society for Cell Biology

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Figure 7. — Suppression of a snaD mutation by down-regulation of both bubA and byrA expressions. (A) When both bubA and byrA genes were down-regulated, the conidiation phenotype caused by the snaD290 mutation was suppressed. Although colonies carrying the snaD290 mutation did not form conidia so that they were brown, the triple mutant produced conidia causing the colony color to be green. (B) Staining of nuclei and septa in the snaD290 single mutant (LB15) and the snaD290; alcA(p)::GFP-bubA; alcA(p)::mCherry-byrA triple mutant (CZA16) grown on media containing glucose. Nuclei were highlighted by arrows, and septa by arrowheads. Scale bar, 10 μm. (C) Quantitative assessment of the number of nuclei in hyphal compartments in the snaD290 single mutant (LB15) and the snaD290; alcA(p)::GFP-bubA; alcA(p)::mCherry-byrA triple mutant (CZA16) grown on media containing glucose. The compartments were grouped together for having <8 nuclei, 8–18 nuclei, or >18 nuclei.