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. 2009 Jun 15;20(12):2885–2899. doi: 10.1091/mbc.E08-12-1160

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© 2009 by The American Society for Cell Biology

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Figure 1. — Suppression of dynein subunit expression using siRNA. Immunoblots show control suppression of lamin A/C from the same experiment, GAPDH as a loading control and the results of transfection with two independent duplexes targeting (A) dynein-1 heavy chain (DYNC1H1), (B) IC2 (DYNC1I2), (C) LIC1 (DYNC1LI1) and LIC2 (DYNC1LI2), (D) Tctex1 (DYNLT1) and rp3 (DYNLT3), (E) LC8 (DYNLL1), and (F) dynein-2 heavy chain (DYNC2H1). Inclusion of duplexes targeting isoforms that are not expressed in these cells provides an additional control and reinforces the specificity of our knockdowns.