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. 2009 Jun 15;20(12):2885–2899. doi: 10.1091/mbc.E08-12-1160

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© 2009 by The American Society for Cell Biology

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Figure 2. — Effects of dynein subunit suppression on dynein integrity. (A–H) Immunoblots show the expression of (A) GAPDH, (B) p50^dynamitin, (C) DHC1, (D) IC2, (E) LIC1, (F) LIC2, (G) LC8, and (H) Tctex in lysates from HeLa cells transfected with siRNA duplexes directed against target proteins as indicated. (I–L) Fractions from 5 to 40% sucrose density gradients from cells depleted of (I) lamin A/C, (J) DHC1, (K) LIC1, or (L) LIC2 were immunoblotted for GAPDH, p50^dynamitin, DHC1, IC2, LIC1, or LIC2 as indicated. Fraction 4 corresponds to 19S as judged by thyroglobulin sedimentation.