Figure 4.
Quantification of disruption of Golgi architecture. Cells depleted of individual dynein subunits using one of two different siRNA duplexes (denoted a and b) were analyzed by automated object analysis as described in Materials and Methods. (A) The localization of GalT was quantified by detecting COPI-labeled structures based on intensity and measuring the intensity of GalT labeling within these areas. (B and C) Histograms showing the number of Golgi particles (>1 μm3) labeled with antibodies to detect (B) COPI or (C) GM130. Error bars, SD; statistical significance, *p < 0.05.