Figure 6.
Quantification of disruption of ERGIC structures and ERES. Cells depleted of individual dynein subunits using one of two different siRNA duplexes (denoted a and b) were analyzed by automated object analysis as described in Materials and Methods. (A) Histogram showing total number of ERGIC-53–positive structures <4 μm3. (B) Histogram showing the total number of ERGIC-53–positive structures >4 μm3. (C) Histogram showing the total number of Sec24C-labeled objects >1 μm3. Error bars, SD; statistical significance, *p < 0.05.