Figure 7.

Effect of dynein subunit suppression on Golgi architecture and transferrin uptake in living cells. Cells stably expressing GRASP65-GFP (green) were depleted of targets as indicated and loaded with AlexaFluor-568-transferrin (red) for 1 h at 37°C followed by imaging also at 37°C. At this time AlexaFluor-568-transferrin labels a significant intracellular pool with little peripheral labeling in controls; peripheral accumulation indicates a defect in centripetal translocation of transferrin-positive endosomes. Symbols indicate where depletion of individual subunits does (plus) or does not (minus) result in peripheral accumulation of transferrin-positive endosomes. Panels show enlargements from entire fields of view shown in Supplementary Figure S6. Bar (all panels), 10 μm.