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. 2009 Jun 15;20(12):2885–2899. doi: 10.1091/mbc.E08-12-1160

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© 2009 by The American Society for Cell Biology

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Figure 9. — LIC2 (DYNC1LI2) and LC8 (DYNLL1) are required for the completion of abscission during cytokinesis. (A) Cells depleted of dynein subunits were processed for immunofluorescence using antibodies to detect α-tubulin (green in right column); cells were counterstained with DAPI to visualize cell nuclei (blue). Bar (all panels), 20 μm. (B) Images were scored by visual inspection for numbers of cells at the stage of abscission (intercellular tubulin bridges) and are expressed as a percentage of cells analyzed (>200 cells from three independent experiments). Error bars, SD, asterisks indicate statistically discernable differences; only LIC2 (DYNC1LI2, p = 0.004), Roadblock1 (DYNLRB1, p = 0.007), and LC8 (DYNLL1, p = 0.04) show statistically detectable differences to lamin sA/ C–depleted controls. (C) Cells depleted of dynein subunits were fixed and immunolabeled to detect phospho-histone H3 (Serine-10). Error bars, SD; asterisk and horizontal bar indicate statistical significance: DHC1 (DYNC1H1) p = 0.002, IC2 (DYNC1I1) p = 0.01, LIC1 (DYNC1LI1) p = 0.01, LIC2 (DYNC1LI2) p = 0.004, Tctex1 (DYNLT1) p = 0.01, and Roadblock1 (DYNLRB1) p = 0.03.