Table 1.
Summary of findings
| GalT localization phenotype (ER-to-Golgi transport) | Golgi scattering phenotype (GM130, GRASP65 labelling) | ERGIC structure phenotype (ERGIC-53 labeling) | Transferrin-labeled endosome phenotype (peripheral distribution) | Abscission phenotype (anaphase bridges) | Phospho-histone H3 labeling phenotype (mitotic progression) | |
|---|---|---|---|---|---|---|
| Dynein-1 heavy chain, DHC1 (DYNC1H1) | NO | YES | YES | YES | NO | YES |
| IC2 (DYNC1LI2) | NO | YES | YES | YES | NO | YES |
| LIC1 (DYNC1LI1) | YES | YES | YES | NO | NO | YES |
| LIC2 (DYNC1LI2) | NO | NO | NO | YES | YES | YES |
| Tctex1 (DYNLT1) | YES | YES | NO | YES | NO | YES |
| rp3 (DYNLT3) | NO | NO | NO | NO | NO | NO |
| Roadblock 1 (DYNLRB1) | NO | YES | NO | YES | YES | YES |
| LC8 (DYNLL1) | YES | NO | NO | YES | YES | NO |
| Dynein-2 heavy chain, DHC2 (DYNC2H1) | NO | NO | NO | NO | NO | NO |
| D2LIC1, LIC3 (DYNC2LI1) | NO | NO | NO | NO | NO | NO |
Defects are summarized here based on visual inspection of images and statistical relevance from automated object quantification. Consequently, as an example, LC8 (DYNLL1) scores negative for Golgi structure because we only see a strong phenotype when using COPI as a marker that also reports ongoing transport between the ER and Golgi. When measured using GM130 or GRASP65-GFP as markers, Golgi structure is not strongly perturbed after LC8 suppression.