Figure 1.

BPAG1e and β4 integrin colocalize at the leading edge of migrating epithelial sheets. A scratch wound was introduced into a confluent monolayer of HEKs. The monolayer was allowed to reclose for 6 h and then was fixed and prepared for indirect immunofluorescence microscopy with antibodies against BPAG1e and β4 integrin as indicated. Top panels, cells migrating into the scratched area; bottom panels, cells distant from the wound site. W and arrow in the phase image in the top panel mark the wound site and direction of migration, respectively. Bar, 20 μm.