Figure 7.

Dynamics of β4 integrin in keratinocytes deficient in BPAG1e. HEKs or HEKCn2s were infected with retrovirus encoding GFP-tagged β4 integrin. FRAP of the arrowed boxes (images of these bleached areas are shown at higher magnification in B) was measured either at the leading edge of cells migrating into a scratch wound (A and B, two top panels; C, black/red lines; arrows indicate direction of migration) or in stationary cells close to or at confluence (A and B, two bottom panels; C, gray/orange lines). Fluorescence recovery is plotted as a percentage of the prebleach levels, allowing for background fading. The graph in C represents mean ± SEM; n = 8.