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. 2009 Jun 15;20(12):2991–3002. doi: 10.1091/mbc.E08-10-1074

Figure 7.

Figure 7.

LIM kinase and cofilin control surface expression of Kv1.2 downstream of ROCK. (A) Overexpression of a dominant-negative form of LIM kinase 1 (D460A) significantly increases steady-state surface Kv1.2 in HEK-K cells as detected by flow cytometry (n = 78; **p < 0.001). (B) LIM kinase 1 (D460A) reduces LPA-induced endocytosis of Kv1.2. Y27632 (10 μM; 30 min) elicits an increase in surface Kv1.2 in the absence or presence of LIM kinase 1 (D460A) (n = 21; p̂ < 0.05, *p < 0.01, **p < 0.001). Inset, in the presence of LIM kinase 1 (D460A), LPA-induced Kv1.2 endocytosis is significantly reduced (n = 21; *p < 0.05). (C) Transient transfection of HEK-K cells with increasing amounts of DNA encoding a constitutively active form of cofilin (S3A) significantly increases steady-state surface Kv1.2 in a concentration-dependent manner (n = 12, *p < 0.01, **p < 0.001).