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. 2009 Jun 30;7(6):e1000147. doi: 10.1371/journal.pbio.1000147

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Mitri et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Knockdown of mtt expression by RNAi in NP4288-GRNs and Gr66a-GRNs suppresses the l-canavanine (Cana)-induced PR gustatory phenotype. Histograms show the percentage of PR of controls (NP4288/+, mtt^f06268/+;UAS-mtt RNAi/+, UAS-mtt RNAi/+, and Gr66a:mtt^f06268/+) and mtt heterozygous flies expressing the mtt RNAi construct in NP4288-positive GRNs (NP4288/mtt^f06268;UAS-mtt RNAi/+) or in Gr66a-GRNs (Gr66a:mtt^f06268;UAS-mtt RNAi/+). Compared to controls, the down-regulation of mtt in NP4288-GRNs or in Gr66a-GRNs suppresses the l-canavanine–induced PR. For all genotypes, l-canavanine does not significantly affect the percentage of PER (see Figure S4A). Behavioral analyses were performed as described in Figure 6. Error bars indicate SEM. Double asterisks indicate significant differences by t-test (p<0.001). (B) Expression of mtt in bitter-sensitive Gr66a-GRNs rescues the PR mutant gustatory phenotype of mtt mutant flies. Histograms show the percentage of PR of control flies (mtt^f06268/+;UAS-mtt/+), mtt mutant flies carrying one copy of each GRN GAL4 (Gr66a:mtt^f06268/mtt^f06268, Gr5a:mtt^f06268/mtt^f06268, and NP1017/+;mtt^f06268/mtt^f06268) and mtt mutant flies expressing mtt in bitter-, sugar-, and water sensitive GRNs (Gr66a:mtt^f06268/mtt^f0 ⁶²⁶⁸;UAS-mtt/+, Gr5a:mtt^f06268/mtt^f06268;UAS-mtt/+, and NP1017/+;mtt^f06268/mtt^f06268;UAS-mtt/+, respectively). Note that only mtt mutant flies expressing mtt in Gr66a-GRNs show a rescue of the l-canavanine–induced PR phenotype.