Figure 2. A septum is open when cytoplasm moves through the septum after laser dissection of a neighbouring compartment.

Compartments before (A, E) and after (B–D, F–H) dissection of a hypha with a closed (A–D) and an open (E–H) septum. Cytoplasmic flow in E–H is indicated by the movement of a vacuole (arrows) through the septum (*). Dots represent the position of the laser.