Table 2. Plugging of the apical septum depends on environmental stress.
| Treatment* | Total number of Septa** | Open Septa | Closed Septa | % Open Septa |
| Hypotonic, 45 min | 5 | 5 | 0 | 100 |
| Hypertonic, 45 min | 5 | 0 | 5 | 0 |
| 20 µg ml−1 nourseothricin | 5 | 1 | 4 | 20 |
| 45°C, 30 min | 5 | 0 | 5 | 0 |
| 45°C, 30 min; 25°C, 15 min | 5 | 4 | 1 | 80 |
| 0°C, 30 min | 5 | 4 | 1 | 80 |
| −20°C, 30 min | 5 | 5 | 0 | 100 |
*
Hyphae were grown in MM medium with glucose. Hypotonic or hypertonic conditions were created by overlaying the agar medium with water and 1 M MgSO4, respectively. Nourseothricin was added to the liquid medium overlaying the agar medium.
**
Hyphae were analysed in two independent experiments.