Table 3.

Lactate dehydrogenase (LDH) assay for cell cytotoxicity

	No. of patients' positive for LDH release from cells stimulated with chlamydial antigens
	Cervical cells			PBMCs
Groups	IncB	IncC	EBs	IncB	IncC	EBs
	n (%)
GI	2(6.45)	3(9.67)	2(6.45)	2(6.45)	2(6.45)	2(6.45)
GII	3(7.89)a	4(10.52)b	3(7.89)c	2(5.26)d	4(10.52)e	5(13.15)f
GIII	4(13.79)	3(10.34)	5(17.24)	4(13.79)	4(13.79)	3(10.34)

Note. Table shows the number of patients (n) positive for LDH release in culture supernatants of stimulated cells. Cervical cells and PBMCs were isolated from patients of GI, GII and GIII and stimulated with chlamydial antigens IncB, IncC and EBs. The number of patients positive for LDH release into culture supernatants post stimulation was noted. Values in parentheses represent corresponding percentages.

^{a, b, c, d, e, f} P not significant as compared to corresponding GI and GIII where, Group I (GI) comprised of healthy fertile women with no CT infection, Group II (GII) comprised of CT-positive fertile women, Group III (GIII) comprised of CT-positive women with fertility disorders.

LDH- Lactate dehydrogenase; PBMCs-Peripheral blood mononuclear cells; IncB- CT Inclusion membrane protein B; IncC- CT Inclusion membrane protein C; CT- Chlamydia trachomatis; EBs-Elementary bodies.

All categorical variables were compared using the χ² test.