Table 6.
IFN-γ ELISPOT assay in IncB or IncC stimulated CD4+ T cell cultures
| GI | GII | GIII | ||||||||||
| IncB (μg/ml) | IncC (μg/ml) | IncB (μg/ml) | IncC (μg/ml) | IncB (μg/ml) | IncC (μg/ml) | |||||||
| 0.5 | 1 | 0.5 | 1 | 0.5 | 1 | 0.5 | 1 | 0.5 | 1 | 0.5 | 1 | |
| Cervical cells |
111 (48–214 |
126 (67–263) |
91 (29–167) |
142 (43–211) |
132a (47–312) |
157b (56–343) |
112c (38–112) |
178d (77–329) |
43 (12–59) |
49 (12–88) |
38 (17–63) |
59 (26–76) |
| PBMCs |
121 (38–200) |
134 (45–212) |
103 (48–176) |
172 (44–176) |
212a (56–273) |
226b (76–301) |
243c (91–322) |
251d (100–316) |
56 (38–97) |
67 (43–115) |
63 (59–108) |
81 (60–149) |
Note. Median number of IFN-γ SFCs per 7.5 × 104 CD4+ T cells at 18 h post stimulation with IncB or IncC (0.5 μg/ml and 1 μg/ml) counted using ELISPOT. Figures in parenthesis depict range of SFCs for each group.
a, b, c, d P < 0.05 Number of IFN-γ in GII compared to respective levels in group III by Mann Whitney U test where, Group I (GI) comprised of healthy fertile women with no CT infection, Group II (GII) comprised of CT-positive fertile women.
Group III (GIII) comprised of CT-positive women with fertility disorders.
IncB- CT Inclusion membrane protein B; IncC- CT Inclusion membrane protein C; CT- Chlamydia trachomatis; EB-Elementary bodies; IL-Interleukin; IFN-γ-Interferon gamma; SFCs-Spot forming cells.