Skip to main content
. Author manuscript; available in PMC: 2009 Jun 12.
Published in final edited form as: Hum Mol Genet. 2007 Jun 21;16(14):1752–1764. doi: 10.1093/hmg/ddm123

Figure 4. Properties of allele-specific binding activity and identification of Phox2a and Phox2b.

Figure 4

(A) Elution profile of nuclear extracts after heparin column chromatography. (B) Allele-selective SCG2 SNP_736 binding activity is enriched in 0.5M NaCl fraction. Equal protein (10μg) was loaded for each fraction. N, no extract; C1, Crude nuclear extract before dialysis; C2, Crude nuclear extract after dialysis; F, Flow-through fraction. (C) Allele-selective binding by PC12 nuclear extract is salt-sensitive. N, no extract; G, G allele probe; A, A allele probe; C, Control probe (TGTACCTAATCACATTTG). (D) EMSA activity is sensitive to factor-specific antibodies. Phox2a antibody produces a modest supershift fraction while pre-incubation with Phox2b antibody neutralizes allele-selective binding for SCG2 SNP_736. Each antibody result was replicated in at least two independent experiments. No, No antibody control; NA, Non-specific antibody (TFIIB).