Figure 3.

Kinetics of 8-OxoG removal in the wild-type MEF cell line. The nonreplicating shuttle vectors, pSΔoriSV-[8-OxoG.C] and pSΔ(ori-p)SV-[8-OxoG.C], were transfected into wild-type MEF cells, recovered after 2- to 12-h incubations, and analyzed for repair of 8-OxoG. Control lanes (C) show mono-modified constructs incubated with (+) or without (−) Fpg. (Left) Southern blots after transfection of plasmids in the wild-type MEF cell line and Fpg-cleavage. (Right) Quantitative analysis of Southern blots. Experimental values are the average of six blots resulting from three transfections with two independent preparations of mono-modified plasmid DNA. Error bars are shown.