Figure 5.

Kinetics of 8-OxoG removal in heterozygous ogg1^+/− cells and homozygous ogg1^−/− cells complemented with murine Ogg1. The nonreplicating shuttle vector, pSΔ(ori-p)SV-[8-OxoG.C], was transfected into cl5 an ogg1^−/− MEF cell line complemented for murine Ogg1 or heterozygous ogg1^−/+ MEFs, recovered after 2- to 12-h incubations, and analyzed for repair of 8-OxoG. Control lanes (C) show mono-modified constructs incubated with (+) or without (−) Fpg. (Left) Southern blots after transfection of plasmids in the MEF cell lines and Fpg-cleavage. (Right) Quantitative analysis of Southern blots. Experimental values are calculated as described in the legend of Fig. 3.