Table 4. Changes in gene expression in the liver as a result of feeding either a chow or a high fat, high cholesterol diet supplemented with 0.25% (wt/wt) CLA isomers (n=8 animals per group). Data are expressed in arbitrary unit relative to β-actin mRNA.
| CLA | ANOVA1 | |||||||
|---|---|---|---|---|---|---|---|---|
| None | c9,t11 | t10,c12 | mean | SED | P value | |||
| LDLr |
Chow HF/HC mean |
1.81 0.42 1.11 |
1.49 0.54 1.01 |
1.17 0.34 0.75 |
1.49 0.43 0.96 |
D C D×C |
0.13 0.16 0.23 |
<0.001 0.082 0.199 |
| ACC |
Chow HF/HC mean |
1.56 0.49 1.02 |
1.27 0.77 1.02 |
1.35 0.38 0.87 |
1.40 0.54 0.97 |
D C D×C |
0.13 0.16 0.22 |
0.001 0.515 0.159 |
| FAS |
Chow HF/HC mean |
1.77 0.37 1.06 |
1.53 0.70 1.12 |
1.55 0.36 0.95 |
1.61 0.47 1.04 |
D C D×C |
0.15 0.18 0.25 |
<0.001 0.647 0.280 |
| LPL |
Chow HF/HC mean |
0.56 1.81 1.19 |
0.54 1.52 1.03 |
0.46 1.89 1.18 |
0.52 1.74 1.13 |
D C D×C |
0.17 0.21 0.30 |
<0.001 0.695 0.569 |
| SREBP-1a |
Chow HF/HC mean |
1.14 1.24 1.19 |
1.62 1.21 1.41 |
1.24 1.05 1.15 |
1.34 1.17 1.25 |
D C D×C |
0.14 0.17 0.24 |
0.154 0.203 0.493 |
| SREBP-1c |
Chow HF/HC mean |
1.14 1.07 1.10 |
1.19 1.30 1.25 |
1.30 1.07 1.18 |
1.21 1.15 1.18 |
D C D×C |
0.11 0.14 0.19 |
0.587 0.569 0.458 |
| SREBP-2 |
Chow HF/HC mean |
2.32 0.72 1.52 |
2.06 1.37 1.71 |
1.57 0.83 1.20 |
1.98 0.97 1.48 |
D C D×C |
0.21 0.25 0.35 |
<0.001 0.133 0.137 |
| ABC-A1 |
Chow HF/HC mean |
0.81 1.39 1.10 |
1.00 1.49 1.24 |
0.80 1.36 1.08 |
0.87 1.41 1.14 |
D C D×C |
0.12 0.14 0.20 |
<0.001 0.447 0.940 |
1
Statistical values were obtained by two-way ANOVA with background diet (D, chow or HF/HC) as one factor and CLA (C, none, c9,t11 or t10,c12) as a second factor. SED: standard error of the difference