Table 1.
Sequenced size-change mutants from heart of old mice
| Mutant ID | Breakpoints (5′ → 3′) | Mutation size* | Location† |
|---|---|---|---|
| 207 | TGATGG/aaacca … ctgagc/GCCGGT | 125 bp | 3,124–3,248 |
| 294 | CGCTGG/aaggcg … ttgaag/TGGCGA | 184 bp | 2,649–2,832 |
| 305 | CGGCGG/agccga … aagcgg/TGAAGT | 528 bp | 1,688–2,215 |
| 180 | GCATCA/tcctct … gcgata/ACGAGC | 866 bp | 1,298–2,163 |
| 239 | GCCGTG/cgctgt … cgccgg/GCAACT | 914 bp | 1,380–2,293 |
| 283 | CTGGAG/gctgaa … gtcgaa/CGCTGG | 1,701 bp | 942–2,642 |
| 192 | GTATGT/tgtgtg … ctttca/CAGATG | 2,346 bp | 171–2,516 |
| 209 | CAGCAA/cgcggc … gccgaa/GCAGCG | 2,846 bp | 5,176–2,675 |
| 99 | GGCGTT/tcatct … nnnnnn/GATCAA | ND | 668-mouse |
| 158 | GGCGAT/gagcga … nnnnnn/ATGAGA | ND | 1,526-mouse |
| 167 | GGATTC/actggc … nnnnnn/TACTTG | ND | 236-mouse |
| 180 | AACGTC/gtgact … nnnnnn/CCATAC | ND | 259-mouse |
| 259 | AGTGGT/caaatg … nnnnnn/TACAGA | ND | 2,805-mouse |
| 273 | TACACG/ctgtgc … nnnnnn/AGGTCG | ND | 1,419-mouse |
| 216 | GGTCAT/ggatga … nnnnnn/ATTGGA | >P1 | 1,319-mouse |
| 233 | GTGGTA/cacgct … nnnnnn/ACTACT | >P1 | 1,415-mouse |
| 288 | TTTCCG/gcacca … nnnnnn/TGGGAT | >P1 | 411-mouse |
| 199‡ | TTCCGC/gaggtg … nnnnnn/CAAAAA | 31 cM | 1,218-chr3 |
| 205‡ | CTGGCC/gtcgtt … nnnnnn/ACCACA | 34 cM | 243-chr4 |
| 256‡ | AGCTGG/cacgac … nnnnnn/TCGCAT | Translocation | 59-chr10 |
| 271‡ | ATTATC/cgaacc … nnnnnn/TATCTG | Translocation | 1,396-chr11 |
Breakpoints 5′ and 3′ of the mutation are shown. The deleted nucleotides are shown in lowercase letters and the remaining nucleotides in capital letters. Underlined sequences indicate direct homology between breakpoints.
Mutation size given in: bp for mutations within the lacZ gene, “>P1” for mutations with breakpoints not present in P1 clones containing the integration sites, and cM or “translocation” for mutations with mapped breakpoints using the backcross panel. ND, not determined.
The nucleotide sequence is numbered according to SYNPUR288V (GenBank accession no. L09147). The numbers correspond to the first and the last deleted base respectively; “chr” stands for the chromosome number the breakpoint was mapped to; “mouse” stands for unlocated breakpoint in the mouse genome.
Approved Mouse Genome Database nomenclature locus names for mapped mouse sequences of mutants 199, 205, 256, and 271 are D3Vij1, D4Vij3, D10Vij1, and D11Vij1, respectively. Other assigned names are Tgn(LacZpl)60aVij, Tgn(LacZpl)60bVij, D4Vij1, and D4Vij2 for cloned upstream region of integration sites A and B and recovered mouse sequences of brain and liver mutants, respectively. Backcross mapping data for these loci are available at www.jax.org/resources/documents/cmdata.