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. 2009 May 13;48(7):734–740. doi: 10.1093/rheumatology/kep091

Fig. 6.

Fig. 6.

ICAM-1 and VCAM-1 mediate adhesion of U937 cells to proximal and distal SSc skin. Stamper–Woodruff in situ assays were performed using frozen skin sections and fluorescent-labelled U937 cells. The percent of maximal binding was defined as the number of adherent cells on the test sections divided by the number of adherent cells on the control sections. A representative image of U937 cell binding to proximal SSc skin in the presence of an IgG isotype control (A) or anti-VCAM-1 antibody (B) is shown. Arrows indicate U937 cells bound to SSc skin. (C) Anti-ICAM-1 antibody-inhibited U937 binding to proximal SSc skin (49% of maximal binding) and distal SSc skin (39% of maximal binding). (D) Anti-VCAM-1 antibody-inhibited U937 binding to proximal SSc skin (59% of maximal binding) and distal SSc skin (65% of maximal binding). (E) The combination of anti-ICAM-1 and anti-VCAM-1 antibody-inhibited U937 binding to proximal SSc skin (39% of maximal binding) and distal SSc skin (39% of maximal binding). Magnification was × 100. Means ± SEM are given. n = the number of patients. P< 0.05 was considered significant.