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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: J Immunol. 2008 May 15;180(10):6816–6826. doi: 10.4049/jimmunol.180.10.6816

FIGURE 7.

FIGURE 7

IL-6 deficiency does not compromise development of antibacterial Abs or NO production. A, Serum samples were collected from IL-6-deficient (○) and wild-type mice (●) 4 wk (top) or 1–4 wk (bottom) after C. rodentium infection. Uninfected mice were used as controls. Anti-C. rodentium titers of IgM (left) and IgG (right) were determined by ELISA. Results are shown as mean ± SEM of the data from six or more mice per group. *, p < 0.05 compared with wild-type mice. B, The colon of IL-6-deficient (○, IL-6−/−) and wild-type mice (●, IL-6+/+) were collected at the indicated times after C. rodentium infection. Total RNA was extracted and analyzed by real-time PCR for iNOS mRNA expression. Data were normalized against GAPDH mRNA expression and are shown as fold change relative to the mRNA levels in uninfected mice. In parallel, colon fragments were cultured for 6 h, and levels of the stable NO breakdown product, nitrite (NO2−), were assayed in the supernatants by the Griess reaction and normalized against dry weight of the tissue fragments. All data are mean ± SEM of four or more mice per group.