Table 1. Primers used for cDNA and genomic PCR and Southern blot probe synthesis.

Opsin category	Primer Name	Sequence
SWS1	SWS1Fw1	5′- AACTACATCYTGGTMAACATCTCC-3′
	SWS1Fw2	5′- TGGGCSTTCYACCTGCAGGC -3′
	SWS1Rev1	5′- GAGTAGGAGAARATGATGATGG-3′
	SWS1Rev2	5′-GAACTGTTTGTTCATGAAGGCG-3′
SWS2	SWS2Fw1	5′-GYACWATTCAATACAAGAARC-3′
	SWS2Fw3	5′-AGCCTTTGGTCTCTGGCTGTG-3′
	SWS2Rev1	5′-AAAGCARAAGCAGAAGAGGAAC-3′
	SWS2Rev4	5′-CCCGTTGTGTACCAGTCTGG-3′
	SWS2AFw1	5′-GTCCACCCGAGTCATAGAGC-3′
	SWS2ARev2	5′-GCCCACGGTTGTTGACAAC-3′
	SWS2-2Fw2	5′-TCTACACCATGGCTGGATTCAC-3′
	SWS2-2Rev1	5′-GATGGTGGTGAATGGAACAGC-3′
RH2	RH2Fw1	5′- AACTTCTAYATCCCGWTGTCC-3′
	RH2Fw2	5′-TGHTCTTCCTGATCTKCACTGG-3′
	RH2Rev2	5′-GTCTCRTCCTCCACCATGC-3′
	RH2Rev4	5′- TGCGGCATGAGTTCCAGTG-3′
	RH2-2Fw1	5′-CAACAGGACGGGCTGGTGAGG-3′
	RH2-2Rev3	5′-ACCCATTCCAATTGTTGCC-3′
RH1	RH1Fw1	5′-ATGAACGGCACAGAGGGACC-3′
	RH1Fw4	5′-GCAGTGCTCATGCGGAGTC-3′
	RH1Rev2	5′-CCTGTTGCTCCATTTATGCAGG-3′
	RH1Rev4	5′-GCTGGAGGACACAGAAGAGG-3′
LWS	Fw100	5′-GATCCCTTTGAAGGACCAAACT-3′
	Fw1a	5′-TCTTATCAGTCTTCACCAACGG-3′
	Gamma Fw1	5′-TGCTATGCAGCAGATAAATTG-3′
	RevEnd	5′-TTATGCAGGAGCCACAGAGG-3′
	Rev8	5′-GCCCACCTGTCGGTTCATGAAG-3′
	RevEx4	5′- CTTCCACTGAACACATCAGG-3′

Primers were used to amplify sequences from A. anableps cDNA and genomic DNA as well as guppy cDNA.