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. 2009 Jun 24;4(6):e5970. doi: 10.1371/journal.pone.0005970

Table 1. Primers used for cDNA and genomic PCR and Southern blot probe synthesis.

Opsin category Primer Name Sequence
SWS1 SWS1Fw1 5′- AACTACATCYTGGTMAACATCTCC-3′
SWS1Fw2 5′- TGGGCSTTCYACCTGCAGGC -3′
SWS1Rev1 5′- GAGTAGGAGAARATGATGATGG-3′
SWS1Rev2 5′-GAACTGTTTGTTCATGAAGGCG-3′
SWS2 SWS2Fw1 5′-GYACWATTCAATACAAGAARC-3′
SWS2Fw3 5′-AGCCTTTGGTCTCTGGCTGTG-3′
SWS2Rev1 5′-AAAGCARAAGCAGAAGAGGAAC-3′
SWS2Rev4 5′-CCCGTTGTGTACCAGTCTGG-3′
SWS2AFw1 5′-GTCCACCCGAGTCATAGAGC-3′
SWS2ARev2 5′-GCCCACGGTTGTTGACAAC-3′
SWS2-2Fw2 5′-TCTACACCATGGCTGGATTCAC-3′
SWS2-2Rev1 5′-GATGGTGGTGAATGGAACAGC-3′
RH2 RH2Fw1 5′- AACTTCTAYATCCCGWTGTCC-3′
RH2Fw2 5′-TGHTCTTCCTGATCTKCACTGG-3′
RH2Rev2 5′-GTCTCRTCCTCCACCATGC-3′
RH2Rev4 5′- TGCGGCATGAGTTCCAGTG-3′
RH2-2Fw1 5′-CAACAGGACGGGCTGGTGAGG-3′
RH2-2Rev3 5′-ACCCATTCCAATTGTTGCC-3′
RH1 RH1Fw1 5′-ATGAACGGCACAGAGGGACC-3′
RH1Fw4 5′-GCAGTGCTCATGCGGAGTC-3′
RH1Rev2 5′-CCTGTTGCTCCATTTATGCAGG-3′
RH1Rev4 5′-GCTGGAGGACACAGAAGAGG-3′
LWS Fw100 5′-GATCCCTTTGAAGGACCAAACT-3′
Fw1a 5′-TCTTATCAGTCTTCACCAACGG-3′
Gamma Fw1 5′-TGCTATGCAGCAGATAAATTG-3′
RevEnd 5′-TTATGCAGGAGCCACAGAGG-3′
Rev8 5′-GCCCACCTGTCGGTTCATGAAG-3′
RevEx4 5′- CTTCCACTGAACACATCAGG-3′

Primers were used to amplify sequences from A. anableps cDNA and genomic DNA as well as guppy cDNA.