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. 2009 Jun 24;4(6):e6028. doi: 10.1371/journal.pone.0006028

Figure 3. Locostatin has no effect on EGF-induced MAPK activity.

Figure 3

(A, B) HeLa cells expressing control or depleted RKIP or (C, D) MEFs expressing wild-type RKIP (RKIP+/+) or (E, F) MEFs not expressing RKIP (RKIP−/−) were serum starved overnight and then pre-treated for 30 min with DMSO or locostatin (concentrations as indicated). EGF (10 ng/ml) was added in the final 5 minutes of incubation. Whole cell lysates (30 µg) were separated on SDS-PAGE gels (12.5%), transferred to nitrocellulose and analysed by immunoblotting with anti-phospho ERK and anti-total ERK antibodies (A, C and E). ERK phosphorylation was assessed by normalizing phospho-ERK levels to total ERK levels as depicted in bar graphs (B, D and F). The results shown are representative of three independent experiments.