Figure 4. Locostatin impairs wound healing in wild-type and depleted RKIP MEFs.

MEFs expressing wild-type (A, B) or depleted (C, D) RKIP were seeded at 0.5×10⁶/ml and grown for 24 h before treatment with either DMSO (control) or locostatin at 20 (A) and (C) or 50 µM (B) and (D). (E) A standardized scratch (wound) was applied to monolayers and digital images were taken at time points indicated. Cell migration was determined as described in materials and methods. Data represents the mean±SEM of eight wounds from two independent experiments. ^*p<0.05, ^**p<0.01 indicates the significance of the change relative to the corresponding sample in the absence of locostatin.