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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: Virology. 2007 Feb 15;363(1):79–90. doi: 10.1016/j.virol.2007.01.015

Table 3.

Binding kinetics of m22 and m24 to gp140R2 and gp14089.6 measured by Biacore.

The experiments were performed as described in the Materials and Methods - m22 and m24 were injected (30 μL/min) at different concentrations (50, 25, 12.5, 6.25 and 3.125 nM) over a CM5 sensor chip, containing captured gp140R2 and gp14089.6 (tethered). The association, ka, and dissociation, kd, rate constants were calculated by fitting both association and dissociation phases with a 1:1 Langmuir global model using BIAevaluation 4.1 software, and the equilibrium dissociation constant, KD, was determined from their ratio.

HIV-1 Isolate ka (105 M−1s−1) kd (10−4 s−1) KD (nM)
m22 m24 m22 m24 m22 m24
gp140R2
(Clade B)		 2.1 1.2 1.6 1.5 0.8 1.3
gp14089.6 (tethered)
(Clade B)		 0.6 0.4 1.6 2.2 2.9 5.2