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. Author manuscript; available in PMC: 2009 Jul 28.
Published in final edited form as: Nature. 2008 Dec 10;457(7229):599–602. doi: 10.1038/nature07586

Figure 2. GNAQQ209L induces MAP kinase activation.

Figure 2

a, Increased expression of pERK in hTERT/CDK4R24C/p53DD melanocytes transfected with GNAQQ209L compared to similar melanocytes transfected with GNAQWT or empty vector. b, Cumulative distribution of mean pixel fluorescence intensity per cell obtained from immunofluorescent detection of pERK (p-values: GNAQQ209L vs. vector). c, Western blot showing increased pERK levels in hTERT/CDK4R24C/p53DD melanocytes expressing Flag-tagged GNAQQ209L compared to cells transduced with Flag-tagged GNAQWT or vector control. NRASQ61R transduced melanocytes are shown as a positive control. * The band migrating just below the Flag band is non-specific reactive band in the lysate.