Skip to main content
. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Virology. 2009 Jan 9;385(1):126–133. doi: 10.1016/j.virol.2008.11.039

Figure 3. Increase in IL-12p40 production in BMDCs following SOCS-1 siRNA nucleofection.

Figure 3

BMDC from Tg and control rats were stimulated with 1 μg/ml of LPS or IFN-γ at 10ng/ml for 48 hrs following SOCS-1 siRNA nucleofection. Figure 3 shows expression of IL-12p40 production by BMDC following siRNA treatment, determined by ELISA as described in the text. IL-12p40 production from SOCS-1 siRNA treated Tg BMDC increased approximately 2-fold following 48 hr of LPS (Figure 3A) or IFN-γ (Figure 3B) stimulation (2424 pg/ml and 322pg/ml), respectively, compared with siRNA-untargeted samples (1494 pg/ml and 143 pg/ml). siRNA-treated controls were 1.5-fold increased (4563 pg/ml and 553 pg/ml), respectively, compared with untreated samples (2974 pg/ml and 367 pg/ml). This figure is representative of two separate experimental studies.