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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Virology. 2009 Jan 9;385(1):126–133. doi: 10.1016/j.virol.2008.11.039

Figure 5. Impaired IL-12Rβ 1 mRNA and secondary IFN-γ expression from Th1 polarized T cells.

Figure 5

Panel A shows IL-12Rβ1 mRNA expression when purified naïve CD4 T cells were stimulated for 3 and 5 days under Th1 conditions. Samples were analyzed in triplicate and data normalized to the expression of 18S ribosomal RNA. IL-12Rβ1 mRNA from Tg CD4+ T cells (n=4) was increased 1.5 and 1.8-fold following 3 and 5 days under Th1 conditions, compared with 4.1 and 7.2 -fold increase in the non-Tg control (n=4) respectively. Panel B shows IFN-γ expression when day 5 Th1 T cells were rested overnight and then pulsed with 1ng/ml rIL-12 for 24 hours as described in the text. Samples were analyzed in duplicate for IFN-γ by ELISA. Interferon-gamma production from Tg (n=5) Th1 conditioned cells was 32 ± 38 pg/ml compared with 528 ± 660 pg/ml from non-Tg control (n=4). * Indicates a significant difference by Mann-Whitney test for samples exhibiting non-normal distribution.