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. 2000 Jul 11;97(15):8415–8420. doi: 10.1073/pnas.140217697

Figure 1.

Figure 1

Diagram of the GAL cluster and the GAL7 promoter. (A) Diagram showing the arrangement of the structural GAL genes. GAL10 and GAL7 are contained within YC10-7. Deletions of the GAL10 poly(A) site result in Δ-55 and Δ-75, respectively. YC10-7 gives rise to monocistronic GAL10 and GAL7 mRNA, respectively (dotted lines below), and is viable on gal medium (Gal+). Δ-55 and Δ-75 give rise to bicistronic mRNA, which does not produce GAL7 mRNA, and are therefore gal sensitive (Gal−). Black boxes represent Gal4p-binding sites. (B) Schematic of the region 260 bp upstream of the GAL7 transcription initiation site (+1). The TATA box and the two Gal4p binding sites are shown (dark boxes); the distances between these elements are indicated below. Also shown are the positions of primers used for in vivo footprinting (P1–P4). (C) Sequences of the two Gal4p binding sites (GAL4-1 and GAL4-2). The center of the roughly symmetrical 17-bp sites is indicated by a vertical, dashed line. The base pairs are numbered sequentially from the center; the central base pair has been assigned the number 0. The protected guanines of the highly conserved outer triplet are in bold and indicated by horizontal bars. They are shown in uppercase and lowercase, depending on the degree of protection. Also indicated are the primers (P1–P4), which were used to visualize the particular strand.