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. 2000 Jul 11;97(15):8415–8420. doi: 10.1073/pnas.140217697

Figure 4.

Figure 4

In vivo footprinting of Gal4p sites on the lower strand. (A) Primer 4: both binding sites GAL4-2 and GAL4-1 are detected. Symbols used are the same as in Fig. 3A. The empty circle in GAL4-2 indicates that residue G 7 is unchanged in all lanes. Protected guanines are shown on the left side. Lane 1, in vitro (vt) control; lanes 2 and 3, Δ-55 in the presence and absence of extra Gal4p, respectively; lanes 4 and 5, YC10-7 in the presence and absence of Gal4p, respectively. (B) Primer 2: detection of GAL4-1. Lane 1, in vitro (vt) control; lanes 2 and 3, YC10-7 in the absence and presence of extra Gal4p, respectively; lanes 4 and 5, Δ-55 in the absence and presence of extra Gal4p, respectively. Note that lane 5 is more than 2-fold overrepresented (relative to lane 4). (C) Quantitation of the GAL4-1 site shown in B. Shown are residues G 7, 6, and −1 (no changes were observed at position −1). Quantitation was performed as described for Fig. 3C.