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. 2000 Jul 11;97(15):8415–8420. doi: 10.1073/pnas.140217697

Figure 5.

Figure 5

The ts mRNA 3′ end CFs affect GAL10 transcription termination. (A) Northern blot of total RNA from gal-induced ts strains (indicated on the top). Strains were grown at the permissive temperature (26°C), induced with gal for 1 h, and then shifted to the nonpermissive temperature (37°C) for 45 min. Because mRNA levels are overall reduced at 37°C, twice the amount of RNA was loaded for all samples at the restrictive temperature (except for Δ-55, where equal amounts were loaded; lanes 1 and 2). GAL transcripts were detected with a probe recognizing GAL10 and GAL7. The filter was prehybridized with total RNA (10 μg/ml hybridization solution) extracted from cells grown in glucose to reduce crosshybridization to rRNA. (B) Northern blot of total RNA from the hpr1 Δgal10-7 strain, transformed with GAL gene plasmids as indicated (lanes 1–4) versus wt (strain N222; lanes 5 and 6).