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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: Am J Respir Cell Mol Biol. 2005 Sep 29;34(2):174–181. doi: 10.1165/rcmb.2005-0280OC

Figure 2.

Figure 2

Recruitment of Nrf 2 and c-Jun to EpRE of GP5 in HNE-stimulated L2 cells. After stimulation with 15 μM HNE, cells were fixed with 1% formaldehyde, lysed; and sonicated to shear chromatin in 0.2- to 0.8-kb fragments, which were then immunoprecipitated with anti-Nrf2 antibody. Nrf2-coprecipitating DNA was analyzed by PCR with specific primers amplifying GP5. (A) Binding of Nrf2 and c-Jun to GP5 EpRE in vivo;(B) negative control.