Fig. 4.
Inhibition of glutathione reductase by BCNU induces PTP1B glutathionylation. After preincubation for 2 h with 100 μM BCNU, cells were immunoprecipitated with the antibody to PTP1B and the immunoprecipitates were separated by electrophoresis and immunoblotted with the antibody to glutathione-protein complexes. The membranes were probed with the antibody to glutathione-protein complexes and then reprobed with the antibody to PTP1B.