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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: Free Radic Biol Med. 2006 Mar 30;41(1):86–91. doi: 10.1016/j.freeradbiomed.2006.03.010

Fig. 4.

Fig. 4

Inhibition of glutathione reductase by BCNU induces PTP1B glutathionylation. After preincubation for 2 h with 100 μM BCNU, cells were immunoprecipitated with the antibody to PTP1B and the immunoprecipitates were separated by electrophoresis and immunoblotted with the antibody to glutathione-protein complexes. The membranes were probed with the antibody to glutathione-protein complexes and then reprobed with the antibody to PTP1B.