Fig. 5.

Adiponectin increased SIRT1 protein expression levels and reversed the inhibition of SIRT1 by LPS or acetate in RKC1 or RAW 264.7 macrophages. A: RKC1 cells were starved in serum-free DMEM overnight, and globular adiponectin (gAcrp) was added at the indicated concentrations for 18 h. SIRT1 protein levels were detected by Western blots with an anti-SIRT1 antibody. B: RKC1 cells were starved in serum-free DMEM overnight, and pretreated with gAcrp (2 μg/ml) for 2 h followed by addition of LPS (100 ng/ml) or acetate (20 mM) for 18 h. SIRT1 protein expression levels were detected by Western blots with an anti-SIRT1 antibody. Data are means ± SE for 3 or 4 replications. *P < 0.05 by 1-way ANOVA. ^aSignificantly different compared with gAcrp-alone group. ^bSignificantly different compared with LPS-alone group.