Fig. 6.

Adiponectin attenuated LPS- or acetate-induced TNF-α production in RKC1. RKC1 cells or RKC1 cells transfected with SIRT1shRNA plasmid were pretreated with gAcrp (2 μg/ml) for 2 h and followed by addition of LPS (100 μM) or acetate (20 mM) for 18 h. TNF-α concentrations in cell culture media were measured by ELISA. Data are means ± SE for 3 or 4 replications. *P < 0.05 by 1-way ANOVA. ^aSignificantly different compared with control. ^bSignificantly different compared with LPS-alone group. ^cSignificantly different compared with acetate-alone group.