Table 5.
States 3 and 4 oxygen uptake of liver mitochondria from rats fed Thr-deficient diets
| Thr, % | State 3 |
State 4 | ||
|---|---|---|---|---|
| NADH Oxidase | Succinate Oxidase | Cytochrome Oxidase | ||
| 0.00 | 29±1* | 70.9±0.9* | 44.8±0.7* | 5.2±0.2* |
| 0.18 | 22.8±0.4* | 92±3 | ND | 4.38±0.08 |
| 0.36 | 38±2* | 93±2 | 54±2 | 3.7±0.2 |
| 0.88 | 42.7±0.3 | 110±9 | 55±1 | 3.6±0.2 |
Values represent means ± SE (n ≥ 5). States 3 and 4 were expressed as nmol oxygen·min−1·mg mitochondrial protein−1. NADH oxidase was assessed with malate-glutamate, succinate oxidase with succinate in the presence of rotenone, and cytochrome oxidase with N,N,N′,N′-tetramethylphenylenediamine and ascorbate in the presence of antimycin; State 4 was taken after State 3 was obtained with malate-glutamate.
*
Significantly different from 0.88% Thr (P < 0.05).