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Characterization of exosome-like vesicles from murine hepatocytes. (a) Cryo-electron micrograph of extracellular vesicles secreted by MLP-29 cells. Bar, 100 nm. (b) Western blot analysis of protein extracts prepared from MLP-29 cells (“C”) or from MLP-29-secreted vesicles (“Ex”). Proteins (10 μg) were analyzed by western blotting using antibodies against Tsg101 (exosome marker) or Grp78 (endoplasmic reticulum marker) proteins.
