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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: J Proteome Res. 2008 Dec;7(12):5157–5166. doi: 10.1021/pr8004887

Table 1. Proteins identified by LC-MS/MS in rat hepatocytes-derived exosomes*.

Secreted proteins
 Serum albumin
 ApoE
 Serum paraoxonases -1 and -3
 Fibronectin precursor
 Fibrinogen beta chain precursor
 Complement C4
 Coagulation factor II / Prothrombin
 Hemoglobin subunits α-1/2
 Clusterin, Kininogen-1 and Serotransferrin
 Polymeric-immunoglobulin receptor
 Dipeptidyl peptidase 4 / CD26
Transmembrane Proteins
 CD63, CD81 and CD82
 Lamp-1
 Membrane-associated progesterone receptor
 Receptor expression-enhancing protein 6
 Sodium/bile acid cotransporter
 Sodium/potassium-transporting ATPase 1α
 Asialoglycoprotein receptor / ASGR
Cytoskeleton-related proteins
 Tubulins: α-2, α-6, β-2A, β-2C, β-3, β-5
 Actin, cytoplasmic 2 and Actin-like protein 2
 Myosin-9, -11
 Moesin and Ezrin
 Cofilin-1
 Dynein heavy chain, cytosolic
Small GTPases
 Ras-related protein: Rab-1A, -1B, -2A, , -7, -11A, -12, -13, -14, -35
 Ras-related protein Rap-1b precursor
 ARF-1, -3, GTPase HRas precursor
Signal transduction
 Guanine nucleotide-binding protein sub beta 2-like 1
 Guanine nucleotide-binding G(I)/G(S)/G(T) beta 2
 Guanine nucleotide-binding G(s) α ,and G(i) -α2
 14-3-3 proteins: β/α, ε, γ, ζ,/δ
 Major vault protein
Adhesion, Membrane transport and fusion
 Lactadherin / Milk fat globule-EGF factor 8
 Annexins: A2, A4, A5, A6
 Clathrin heavy chain
 Coatomer subunits beta and beta’
 Transmembrane emp24 domain-containing protein 2
 PDC6CI / AIP1/ Alix
 Cation-dependent mannose-6-phosphate receptor
 Transitional endoplasmic reticulum ATPase
Protein synthesis and post-traductional
 40S ribosomal protein SA, S3, S4(X), S6
 60S ribosomal protein L6 and L7
 EF1α1, EF1α2 and EF2
Protein Folding
 Heat shock proteins: HS70L, HSP71, TRAP1, HSP90-α and -β
 Grp78
 Calnexin
 T-complex protein 1 subunits α, β, δ, ε, γ
 Protein disulfide-isomerase A3
 Calregulin / Calreticulin
Protein Processing and degradation
 Aminopeptidase N / CD13
 Ubiquitin
Lipid Metabolism
 Fatty acid synthase and ATP-citrate synthase
 Long-chain specific acyl-CoA
 Long-chain-fatty-acid-CoA ligase -1 and -6
 Peroxisomal bifunctional enzyme
 Acyl-coenzyme A oxidase 2, peroxisomal
 Hydroxymethylglutaryl-CoA synthase, mito.
 Fatty aldehyde dehydrogenase
Carbohydrate and Amino-acid Metabolism
 Alpha-enolase
 Fructose-1,6-bisphosphatase 1, aldolase A
 Glyceraldehyde-3-phosphate dehydrog.
 Pyruvate kinase isozymes R/L
 Pyruvate carboxylase, mitochondrial
 Phosphoglycerate kinase 1
 4-trimethylaminobutyraldehyde
 L-lactate dehydrogenase A chain
 Malate dehydrogenase, cytoplasmic
 Cystathionine gamma-lyase
 C-1-tetrahydrofolate synthase, cytoplasmic
 10-formyltetrahydrofolate dehydrogenase
 Formimidoyltransferase-cyclodeaminase
 Adenosylhomocysteinase
 Betaine--homocysteine S-methyltransferase
 Cysteine sulfinic acid decarboxylase
 Arginase-1
 Argininosuccinate synthase and lyase
 Carbamoyl-phosphate synthase
Hormone Metabolism
 3-oxo-5-beta-steroid 4-dehydrogenase
 Corticosteroid 11-beta-dehydrogenase isozyme 1
 Retinol dehydrogenase -2, -3
 All-trans-retinol 13,14-reductase precursor
Xenobiotics / Endogenous compounds Metabolism
 Cytochromes P450: 2A1, 2B3, 2C11, 2D1 2D3, 2D10, 2D18, 2D26
 UDP-glucuronosyltransferases: 2B2, 2B3 and 2B5
 NADPH-cytochrome P450 reductase
 Alcohol dehydrogenase 1 and Epoxide hydrolase 1
 Microsomal glutathione S-transferase 1
 Sulfotransferase 1A1
 Thiosulfate sulfurtransferase
 Dimethylaniline monooxygenase [N-oxide-forming]
Nuclear Proteins
 Histones: H2A-1, H2B-1, H2B-1A, H4
 Importin beta-1 subunit
Miscellaneous
 ATP synthase subunits α and β
 Cytochrome b5
 Ferritin heavy chain
 Ferritin light chain 1
*

Proteins listed appear at least in two independent exosomes preparations or in exosomes from other sources previously reported (see Table S1 for details).

Presence detected also by western blotting (see Figure 4).