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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Differentiation. 2008 Oct 31;77(1):70–83. doi: 10.1016/j.diff.2008.09.003

Figure 6. En face immunolabelling of AQP0 and WGA in isolated lens fiber cells.

Figure 6

Layers of fiber cells were sequentially peeled off the lens to yield isolated cells from the periphery (A), deeper cortex (B), and mature lens fiber cells from the inner cortex (C). Cells were labelled with the general membrane label WGA (green, left panel) and an AQP0 antibody (red, middle panel). Images shown are single optical sections taken to capture the broad side surface of the cell. Arrowheads indicate ball-and-socket joints located to the narrow sides of fiber cells, while asterisks highlight WGA-negative regions indicative of large broad side gap junction plaques. The two channels are merged in the right panel. Scale bar = 5 μm.