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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: J Mol Biol. 2007 Jul 3;372(2):369–381. doi: 10.1016/j.jmb.2007.06.073

Figure 4.

Figure 4

Analysis of p51 mutants containing F130W. M7 proviral DNA was transfected into 293T cells alone or together with wild-type or mutant vpr-p51/p66 and vpr-IN expression plasmid DNAs. (a) Virion incorporation and proteolytic processing of trans-heterodimeric RT. Immunoblot analysis of transfection-derived virions obtained with M7 plus vpr-p51/p66, vprΔp51/p66, vpr-p51(F130W)/p66, and vpr-p51(T58S/F130W)/p66, using the RT-specific monoclonal antibody 8C4 (upper panel) and a CA-specific monoclonal antibody 183-H12-5C (lower panel). (b) Immunoblot analysis of the expression of p66 and α-tubulin in the transfected 293T cells. Samples were analyzed with a mixture of monoclonal antibodies against the RNase H domain of HIV-1 RT (7E5) and α-tubulin.