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PR stability. (a) Sensitivity to urea: wild-type HIV-1 PR (filled circles) (UC50 = 1.22 M), G94S (open circles) (UC50 = 0.63 M), and T96S (filled triangles) (UC50 = 0.74 M). (b) Dimer dissociation: wild-type HIV-1 PR (filled circles) (Kd < 0.37 nM, no dissociation observed), G94S (open circles) (Kd = 26.3 ± 11.7 nM), and T96S (filled triangles) (Kd = 4.94 ± 1.07 nM).
