Figure 2.




Blood counts and human cell engraftment after busulfan conditioning. (A–C): PB counts of NOD/SCID IL2Rγnull mice after busulfan conditioning and transplantation of human cells. Complete blood counts were determined from day 0 to day 50 post transplantation. Shown are unselected CB (1×108 cells, busulfan 25mg/kg, n=7 mice), CB CD34 (2×106 cells, busulfan 50mg/kg, n=8), PB CD34 (2×106 cells, busulfan 50mg/kg, n=5), and 1Gy PB (2×106 cells, 1Gy, n=5). (D): Percentage of circulating human CD45+ cells in the early engraftment period. PB was analyzed for the percentage of cells of human origin by flow cytometry for CD45 positivity from day 0 to day 50 post transplantation. Shown are unselected CB (1×108 cells, busulfan 25mg/kg, n=7 mice), CB CD34 (2×106 cells, busulfan 50mg/kg, n=8), PB CD34 (2×106 cells, busulfan 50mg/kg, n=5), and PB 1Gy (2×106 cells, 1Gy, n=5). CB, cord blood; PB, peripheral blood; unsel, unselected; WBC, white blood cell.