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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Protein Expr Purif. 2009 Feb 21;66(2):121–130. doi: 10.1016/j.pep.2009.02.012

Figure 2. Thermal Denaturation of Wild-type and Δ41-52 Human Prolactin.

Figure 2

1.0 μM solution of either wild-type hPRL or Δ41-52 hPRL was placed into heated cuvets in a LS55 Perkin/Elmer spectrophotometer in 10 mM Tris pH 7.5 and 150 mM NaCl. Temperatures were increased at a rate of 1C° per minute from 25C° to 95C°. The absorbance was measured at 280 nm at 5C° increments. When 95C° was achieved the temperature of Δ41-52 hPRL was returned to 25C°; the reduction in absorbance observed between 90 and 95C° appears to be a result of precipitation of the denatured protein. This same reduction in absorbance is retained when the temperature is returned to 25C° suggesting that the protein precipitate does not return to a solution. A basal thermal-induced rate of absorbance change was determined at low temperatures and subtracted from the measured data.