Table 1.

Structural Changes in the Sequence of Residues 41-52 of Human Prolactin.

	Structural Change	Yield		Agonist Activity
Protein	Residues 35-55 with deletions or replacements underlined	Monomer yield	Monomer percentage	ED50 (nM)	Fold decrease
WT hPRL	EMFSEFDKRYTHGRGFITKAI	15.4 mg	90%	0.23	1
Δ41-52 hPRL	EMFSEFKAI	9.4 mg	42%	702.42	3041
G2-ΔhPRL	EMFSEFGGKAI	2.7 mg	39%	5.25	23
G3-ΔhPRL	EMFSEFGGGKAI	7.9 mg	40%	18.91	82
G4-ΔhPRL	EMFSEFGGGGKAI	13.5 mg	57%	1277.16	5312
G5-ΔhPRL	EMFSEFGGGGGKAI	11.4 mg	67%	1.36	6
A2-ΔhPRL	EMFSEFAAKAI	6.2 mg	34%	163.38	707
SPGG-ΔhPRL	EMFSEFSPGGKAI	8.7 mg	49%	0.44	2
DGFIT-ΔhPRL	EMFSEFDGFITKAI	17.5 mg	52%	45.94	207
DIT-ΔhPRL	EMFSEFDITKAI	13.4 mg	70%	47.93	199
G129R hPRL	-------	18.3 mg	90%	5.99	26