Table 2.
Flow Cytometry Data for Hormone-treatment of Human Jurkat Cells (representative of three studies)*.
| Wild-type human prolactin | G129R human prolactin | Δ41-52 human prolactin | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dose | Time | % Live | % Apop. | % Necro. | % Total | % Live | % Apop. | % Necro. | % Total | % Live | % Apop. | % Necro. | % Total |
| 0.0 nM | 24 hrs | 93.9 | 1.9 | 3.8 | 99.6 | 93.9 | 1.9 | 3.8 | 99.6 | 93.9 | 1.9 | 3.8 | 99.6 |
| 48 hrs | 94.6 | 0.9 | 4.2 | 99.7 | 94.6 | 0.9 | 4.2 | 99.7 | 94.6 | 0.9 | 4.2 | 99.7 | |
| 72 hrs | 95.8 | 0.8 | 3.2 | 99.8 | 95.8 | 0.9 | 3.2 | 99.9 | 95.8 | 0.8 | 3.2 | 99.8 | |
| 96 hrs | 94.1 | 2.6 | 3.2 | 99.9 | 94.1 | 2.6 | 3.2 | 99.9 | 94.1 | 2.6 | 3.2 | 99.9 | |
| 0.1 nM | 24 hrs | 92.7 | 2.0 | 5.0 | 99.7 | 90.2 | 1.9 | 7.8 | 99.9 | 88.2 | 2.4 | 9.2 | 99.8 |
| 48 hrs | 92.8 | 1.3 | 5.3 | 99.4 | 93.6 | 1.0 | 5.2 | 99.8 | 77.1 | 9.4 | 12.4 | 98.9 | |
| 72 hrs | 95.2 | 1.0 | 3.7 | 99.9 | 95.9 | 1.1 | 2.8 | 99.8 | 58.7 | 7.1 | 33.3 | 99.1 | |
| 96 hrs | 91.0 | 4.1 | 4.8 | 99.9 | 92.9 | 3.1 | 3.6 | 99.6 | 54.5 | 5.5 | 38.6 | 98.6 | |
| 1.0 nM | 24 hrs | 90.7 | 2.0 | 7.1 | 99.8 | 89.7 | 1.9 | 8.2 | 99.8 | 85.6 | 2.4 | 11.7 | 99.7 |
| 48 hrs | 90.7 | 1.6 | 7.2 | 99.5 | 94.9 | 1.2 | 3.7 | 99.8 | 75.1 | 9.5 | 13.5 | 98.1 | |
| 72 hrs | 92.5 | 1.2 | 6.2 | 99.9 | 95.6 | 1.0 | 3.3 | 99.9 | 63.2 | 6.0 | 30.7 | 99.9 | |
| 96 hrs | 91.9 | 2.5 | 5.4 | 99.8 | 92.6 | 2.5 | 4.4 | 99.5 | 27.0 | 7.2 | 61.3 | 95.5 | |
| 10 nM | 24 hrs | 91.3 | 1.5 | 6.9 | 99.7 | 87.9 | 1.9 | 10.2 | 100 | 83.4 | 2.5 | 14.0 | 99.9 |
| 48 hrs | 92.7 | 1.4 | 5.5 | 99.6 | 94.3 | 1.3 | 4.2 | 99.8 | 74.2 | 11.1 | 13.9 | 99.2 | |
| 72 hrs | 95.1 | 0.8 | 4.0 | 99.9 | 95.4 | 0.9 | 3.6 | 99.9 | 49.5 | 7.1 | 42.8 | 99.4 | |
| 96 hrs | 91.0 | 2.0 | 6.8 | 99.8 | 93.6 | 2.5 | 3.6 | 99.7 | 57.7 | 4.9 | 37.6 | 100.2 | |
| 100 nM | 24 hrs | 92.0 | 1.6 | 6.2 | 99.8 | 91.3 | 1.6 | 6.9 | 99.8 | 88.8 | 1.7 | 9.4 | 99.9 |
| 48 hrs | 91.3 | 1.3 | 7.2 | 99.8 | 92.5 | 1.2 | 6.2 | 99.9 | 79.1 | 7.7 | 12.6 | 99.4 | |
| 72 hrs | 94.1 | 1.3 | 4.3 | 99.7 | 95.7 | 1.3 | 2.8 | 99.8 | 58.3 | 6.7 | 34.5 | 99.5 | |
| 96 hrs | 93.4 | 1.8 | 4.5 | 99.7 | 93.9 | 2.6 | 3.3 | 99.8 | 68.6 | 5.1 | 25.5 | 99.2 | |
| 0.5 μM | 24 hrs | 91.6 | 2.1 | 6.1 | 99.8 | 89.5 | 1.9 | 8.4 | 99.8 | - | - | - | - |
| 48 hrs | 90.8 | 1.7 | 7.3 | 99.8 | 92.2 | 2.3 | 6.2 | 100.7 | - | - | - | - | |
| 72 hrs | 89.6 | 2.9 | 7.3 | 99.8 | 95.2 | 0.6 | 4.0 | 99.8 | - | - | - | - | |
| 96 hrs | 88.6 | 2.6 | 8.6 | 99.8 | 93.8 | 2.3 | 3.6 | 99.7 | - | - | - | - | |
| 1.0 μM | 24 hrs | 88.5 | 2.4 | 9.0 | 99.9 | 91.6 | 1.8 | 6.5 | 99.9 | 85.1 | 2.1 | 12.6 | 99.8 |
| 48 hrs | 92.0 | 1.8 | 5.8 | 99.6 | 94.1 | 3.2 | 4.6 | 101.9 | 73.3 | 10.3 | 15.6 | 99.2 | |
| 72 hrs | 93.0 | 1.2 | 5.6 | 99.8 | 95.7 | 1.0 | 3.3 | 100 | 65.7 | 5.0 | 28.6 | 99.3 | |
| 96 hrs | 92.0 | 2.4 | 5.6 | 100 | 93.0 | 3.2 | 3.7 | 100 | 83.3 | 2.3 | 13.7 | 99.3 | |
*
Percent of the total cells recorded in quadrants that represent living cells, loss of mitochondrial membrane potential (apoptosis), loss of mitochondrial potential and increased PI permeability (necrosis), and total percentage of cells in the above three quadrants.